Wright's stain is a histological stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining).[1] The May–Grunewald stain, which produces a more intense coloration, also takes a longer time to perform. Urine samples stained with Wright's stain will identify Eosinophils which can indicate Interstitial nephritis or Urinary tract infection.
Applications of Wright Stain :-
1. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates which are examined under a light microscope.
2. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when infections are suspected.
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