Sulphite (Sulfite) Test Kit indicator TK - 15

Sulfite, Titrimetric Method(0-100ppm)

The test  for sulfite is based on the colour change end point reaction. At the end point reagent combines with the indicator to form a blue color. 

Apparatus Required

Buret, automatic, 25 ml    -  1 ( Sample Tube, Graduated, 25 mL, plastic w/cap and white dot )
Casserole porcelain, 210 ml  -  1
Cylinder, graduated, 50 ml  -  1
Measuring dipper (plastic)  -  1
Stirring rod, glass    -  1 

Chemicals Required

TK - 13 ::: RXSOL-62-5503-002
TK - 14 ::: RXSOL-62-5503-003
TK - 15 ::: RXSOL-62-5503-004  ( 1 ml =0.5 mg SO3 )
 

Procedure for Test

The water sample should be freshly obtained with as little contact as possible with air. Do not filter the sample, but cool it to room temperature (70 to 800F).
NOTE: 

Sample must be cooled to less than 100ºF (38ºC) to prevent high test results. 

Sample must be protected from air contact while cooling to prevent low test results. 

1. Measure 50 ml of the water sample with the graduated cylinder . For error free result Collect water to be tested in a clean, preferably large-mouthed, bottle to overflowing. Immediately cap and cool to room temperature. 

2. Add three or four drops of TK - 13 ::: RXSOL-62-5503-002  to the sample Swirl to mix. Sample should turn red. 

3. Add  TK - 14 ::: RXSOL-62-5503-003 Powder a dipper at a time, swirling after each dipper, until color changes from red to colorless. Add 2 more dippers. Swirl until dissolved.  ( Note : Use the plastic dipper to add TK - 14 ::: RXSOL-62-5503-003 to the sample. Add only one measure at a time and stir thoroughly between each addition of TK - 14 ::: RXSOL-62-5503-003. All the particle of TK - 14 ::: RXSOL-62-5503-003 may not dissolve and this may create a slight haze in the sample. Continue to add the TK - 14  in this manner until the red color disappears. It is not necessary that the sample solution be exactly neutralized, only that the sample turn colorless. When the sample is colorless, add one additional measure of TK - 14 ::: RXSOL-62-5503-003 and stir. )

4. Add TK - 15 ::: RXSOL-62-5503-004 Reagent dropwise, swirling and counting after each drop, until color changes from colorless to a faint but permanent blue. Always hold bottle in vertical position. 
This color change is taken as the endpoint. Record the ml of TK - 15 ::: RXSOL-62-5503-004 solution used. 

Calculation of Results

FORMULA:    ppm sulfite as SO3  = ml  of TK - 15 ::: RXSOL-62-5503-004  x       500    /  ml SAMPLE  

                        Using a 50 ml sample, sulfite, in parts per million as SO3  is equal to the ml of TK - 15 ::: RXSOL-62-5503-004 required multiplied by 10. 

Limitations of Test :::

This method is rapid and adaptable to field determinations.  It is affected by any oxidizable substances in the water such as organic matter sulfides and nitrites.  The presence of these interfering substances will cause the sulfite content obtained from this titration to by shown as a higher value than actually exists.